Table 5. Effective discrimination of three bar-coded cell-based assays.
| FPNLSFP-markedLNCaP-C4-2 cell lines | # objectstotal | # objects inem1/em2 = | # objects inem1/em2 = | # objects inem1/em2 = | # otherobjects |
| 0.4841–0.6354* | 0.6436–0.8299* | 0.9331–1.2003* | |||
| mCherryNLSmCherry | 22,551 | 22,466 | 62 | 0 | 23 |
| (100%) | (99.62%) | (0.27%) | (0.00%) | (0.10%) | |
| mRaspberryNLSmKate2 | 19,131 | 7 | 19,108 | 0 | 16 |
| (100%) | (0.04%) | (99.88%) | (0.00%) | (0.08%) | |
| mPlumNLSmPlum | 39,345 | 0 | 7 | 39,318 | 20 |
| (100%) | (0.00%) | (0.02%) | (99.93%) | (0.05%) |
*
Range of em1/em2 ratios within which the segmented nuclei were assigned, defined by mean +/−3 sd in em1/em2 ratios characteristic of each cell line.