A, A calibration curve of LAIR-2 ELISA was performed. The plotted OD values were obtained with serial dilution of LAIR-2-Fc protein. In addition, no relevant cross-reaction with soluble LAIR-1-Fc was detectable. A representative experiment of three is shown. Bars represent standard deviation. B, Serum LAIR-2 is measurable both in healthy donors and patient with ATD, otherwise ATD had far higher circulating LAIR-2 levels. C, LAIR-2 ELISA on 59 sera of GD, 20 of GD patients one month after radio-iodine treatment, 21 HT patients, 10 of patients with MNTG and 25 healthy donors. Samples were diluted 1∶10 and tested in triplicate (deviation between triplicates <10%; detection limit 0.1 ng/ml).