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. 2013 May 14;8(5):e63333. doi: 10.1371/journal.pone.0063333

Figure 3. Blunted PKA-mediated signaling in HPRT-deficient MN9D cells.

Figure 3

(A), Immuno-blot analysis of phospho-PKA-substrate expression and p-Syn I (Ser9) in response to forskolin exposure. (B), quantification phospho-synapsin relative to the total amount of protein measured as beta-actin. Error bars represent mean ± SEM of duplicate measurements of two independent experiments (n = 4). The asterisk (*) represents statistical significance between forskolin treated cells (p<0.05 t-test). (C&D), Effect of 6-bnz-cAMP on phospho-PKA-substrate and p-Syn I (Ser9) expression in HPRT-deficient MN9D cells. Data show reduced expression of phospho-PKA-substrates including p-Syn I (Ser9) in HPRT-deficient MN9D cells, after 6-bnz-cAMP treatment. The quantification of p-Syn relative to beta-actin is seen in figure D. Error bars represent mean ± SEM of duplicate measurements of two independent experiments (n = 4). The asterisk (*) represents statistical significance between 6-bnz-cAMP treated cells (p<0.05 t-test).