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. 2013 May 14;8(5):e64239. doi: 10.1371/journal.pone.0064239

Figure 2. The expression of HXS1 is repressed by glucose.

Figure 2

The qRT-PCR method was used to detect the change in transcription levels of HXS1 and HXS2 under growth conditions with different glucose levels. H99 cells were grown on YPG (no glucose) liquid medium, then switched to medium with 0.01%, 0.1%, 1% or 2% glucose (A), or grown on YPD and switched to medium with lower glucose levels (1%, 0.1%, 0.01% or YPG) (B). Cells were collected after 2 hr incubation and RNA prepared for qRT-PCR. Values are expressed as relative expression (log2) of the HXS1 or HXS2 gene, normalized to the GAPDH gene endogenous reference. The changes in gene transcription levels were related to 0-hr time point (H99 overnight liquid cultures on either YPG (A) or YPD (B)). The error bars showed standard deviations of three repeats.