Table 2. Gene specific primers used for RT-PCR analyses: bNBC1 (brain NBC1), kNBC (kidney NBC1), NBC2 (NBCn-1b) and NBC3 (NBCn1-d) isoform specific primers were designed from published sequences.
Gene | Primer | Position | Accession number |
NBCe1A | Sense: 5′-GGCACAGAGAGAGGAGGCTT-3′ Antisense: 5-TGTCTTCCCAATGTCAGCCAG-3′ | 17–36 593–613 | AF027362 |
NBCe1B | Sense: 5′-ACTGGAGGAGCGACGGAAG-3′ Antisense: 5′-TGTCTTCCCAATGTCAGCCAG-3′ | 27–45 730–750 | AF210250 |
NBCn1 | Sense 5′-ATCTACCTCCGCTATGTCC-3′ Antisense: 5′-ACTCACAGGCTTTTCAGGGC-3′ | 3424–3442 3881–3900 | AF080106 |