Figure 3.
Results from qPCR to determine relative RNA levels of membrane HCmu, indicated in fold-change on the Y-axis relative to the reference sample. Mean fold-change values with SE. N values indicated underneath each group on X-axis. Reference sample (in black) was mature, non-spawning O. mykiss. All other groups are O. nerka. SW-MB: salt-water, pre-spawning, at Main Bay (in vertical stripe). Juvenile (white): pre-smolts from Trail Lake Hatchery. Kenai run: in grey, note a dotted arrow on X-axis. SW-MoK: salt-water, pre-spawning, at Mouth of the Kenai; FW-MSR: fresh-water, pre-spawning, at confluence of Mouth of the Kenai and Moose River; SS-Pre: fresh-water, pre-spawning, at Quartz Creek; SS-Post: fresh-water, post-spawning, at Quartz Creek. A. Anterior kidney. B. Spleen. C. Posterior Kidney.