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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Free Radic Biol Med. 2013 Feb 14;60:10.1016/j.freeradbiomed.2013.02.003. doi: 10.1016/j.freeradbiomed.2013.02.003

Figure 1. ATN-224 induced cell death in WEHI7.2 and WEHI7.2 variant cells.

Figure 1

A. Immunoblot showing Bcl-2 (26 kDa) protein levels in WEHI7.2 (W), Hb12 (H) and 200R (R) cells. Immunoblot showing actin (42 kDa) protein levels to demonstrate similar loading. B. Cell viability in WEHI7.2 and WEHI7.2 variant cells treated with nanomolar concentrations of ATN-224. C. Caspase 3 activity measured in WEHI7.2 (24 h), Hb12 (18 h) and 200R (30 h) cells treated with vehicle (C) or ATN-224 (A). D. Propidium iodide uptake in WEHI7.2 (30 h), Hb12 (30 h) and 200R (36 h) cells treated with vehicle (C) or ATN-224 (A). All values are mean + SEM (n ≥). *, significantly different from vehicle treated control cells (p ≤ 0.05).