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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Free Radic Biol Med. 2013 Feb 14;60:10.1016/j.freeradbiomed.2013.02.003. doi: 10.1016/j.freeradbiomed.2013.02.003

Figure 3. ATN-224 induced cell death is peroxynitrite-dependent.

Figure 3

A. Cell viability in WEHI7.2 and WEHI7.2 variant cells treated with vehicle, ATN-224, 50 nM MnTE-2-PyP5+, 1 μM (OH)FeTM-4-PyP4+, or a combination of ATN-224 and either 50 nM MnTE-2-PyP5+ or 1 μM (OH)FeTM-4-PyP4+ for 48 h. B. Nitrotyrosine levels in WEHI7.2 and WEHI7.2 variant cells treated with vehicle (C) or ATN-224 (A) for 24 h. C. Cell viability in WEHI7.2 and WEHI7.2 variant cells treated with vehicle, ATN-224, 100 μM MnTBAP3− or a combination of ATN-224 and 100 μM MnTBAP3− for 48 h. D. Cell viability in U937 and Molt-4 cells treated with vehicle, ATN-224, 50 nM MnTE-2-PyP5+, 1 μM (OH)FeTM-4-PyP4+, or a combination of ATN-224 and either 50 nM MnTE-2-PyP5+ or 1 μM (OH)FeTM-4-PyP4+ for 72 h. E. Cell viability in U937 and Molt-4 cells treated with vehicle, ATN-224, 50 μM MnTBAP3− or a combination of ATN-224 and 50 μM MnTBAP3− for 72 h. All values are mean + SEM (n ≥). *, significantly different from ATN-224 treated control cells (p ≤ 0.05).