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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Free Radic Biol Med. 2013 Feb 14;60:10.1016/j.freeradbiomed.2013.02.003. doi: 10.1016/j.freeradbiomed.2013.02.003

Figure 4. ATN-224 effects on the mitochondria.

Figure 4

A. Immunoblot showing Bcl-2 (26 kDa) protein levels in WEHI7.2 and WEHI7.2 variant cells treated with vehicle or ATN-224 before and after caspase 3 activation. B. CcOX activity in WEHI7.2 and WEHI7.2 variant cells treated with vehicle (C) or ATN-224 (A) for 12 h. KCN (K) included as a positive control. C. Immunoblots showing CcOX subunits Va (16.8 kDa) and Vb (13.7 kDa) protein levels in WEHI7.2 and WEHI7.2 variant cells treated with vehicle control or ATN-224. Immunoblots showing actin (42 kDa) protein levels to demonstrate similar loading. D. JC-1 aggregates in WEHI7.2 and WEHI7.2 variant cells treated with vehicle (C), 50 nM MnTE-2-PyP5+ (Mn), ATN-224 (A) or a combination of 50 nM MnTE-2-PyP5+ and ATN-224 (MnA) for 6 h. All values are mean + SEM (n ≥). *, significantly different from vehicle treated control cells (p ≤ 0.05).