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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Plant J. 2013 Mar 25;74(4):626–637. doi: 10.1111/tpj.12152

Figure 6.

Figure 6

Oxylipin-mediated induction of YUCCA gene expression. Quantitative real time reverse transcriptase PCR for YUC8 and YUC9 gene expression in wild type (a), and both yuc8 and yuc9 (b), as well as coi1 (c) mutant seedlings after treatment under variable conditions. Seedlings were either treated with a 50 μM MeJA, 50 μM OPDA, 10 μM coronatine or control solution (½ MS media, 0.5% MeOH (v/v)). The duration of induction varied between 30 to 240 min (a) and 2 h (b,c). Additionally, a long-term treatment with 20 μM MeJA in plates was conducted (b). Then, seedlings were harvested, total RNA extracted, and transcript levels examined by qRT-PCR. Relative expression levels of the genes are normalized to levels in mock controls or untreated control samples and represent the average of three biological replicates, with error bars denoting the standard error. [Student’s t test; ** P < 0.01]. Mean values within a graph are significantly different [P < 0.05] where superscript letters differ.