Figure 7.
Induction of YUC gene expression. (a–c) GUS staining of pYUC8::GUS and pYUC9::GUS promoter reporter lines after treatment with MeJA. (a) YUC9 promoter activation 2 hours after application of 10 μL 1 mM MeJA on a single leaf (arrow). Promoter activity in primary root segments of seven-day-old pYUC8::GUS and pYUC9::GUS lines 2 hours after treatment with either a mock control (b) or with a solution containing 50 μM MeJA (c). [Scale bars: 1 cm (a–c)]. (d) Pattern of GUS activity in four-week-old sterilely grown wild type, AOS-promoter GUS line 4-1 (AOS) (Kubigsteltig et al., 1999), pYUC8::GUS (YUC8) and pYUC9::GUS (YUC9) lines 12 hours after wounding with sterile forceps (arrows). [Scale bars: 20 μm (d)].