Fig. 6.

LPS is required for H₄R-dependent sensitivity in a mouse asthma model. Wild-type mice (n = 5–8 per group) were sensitized to ovalbumin i.p. on Days 0 and 14 before repeat aerosol exposure to ovalbumin on Day 21 through 24. The ovalbumin used was either LPS-free (No LPS) or spiked with 1 ng of LPS. Vehicle (Veh) or JNJ 7777120 (JNJ) was administered p.o. 15 min prior to each challenge. a The number of eosinophils was calculated from bronchoalveolar lavage fluid collected 24 h after the last OVA challenge. b IL-13 levels were measured from bronchoalveolar lavage fluid. Statistical significance was determined by one-way ANOVA with post hoc Bonferroni’s test; ns indicated not significant