Figure 3.

Inhibition by 1 μM simvastatin of (A) ADP or (B) FCCP-stimulated oxygen consumption supported by complex I substrates was prevented by mevalonate, Co-Q10 or L-carnitine. Skeletal muscle samples were incubated in MiR05 medium in the presence of 1 μM simvastatin, or DMSO for 1 h containing 100 μM mevalonate, 10 μM coenzyme Q10, or 1 mM L-carnitine. ADP (400 μM) or 1 μg/mL oligomycin plus 0.2 μM FCCP were added and oxygen consumption was supported by 10 mM glutamate plus 5 mM malate. Since the control and 1 μM simvastatin bars were not statically different in Figure 1 and panel (A), we used a general averages for controls and 1 μM simvastatin in both figures. ^*p < 0.05 vs control by one-way analysis of variance. N = at least 6 independent experiments.