Figure A1.

G + C content (%) frequency plot of random shotgun sequence reads (Sanger) obtained from Washburn Springs (WS_18) sediments rich in elemental sulfur and pyrite. Phylogenetic classification of each sequence read (∼800 bp) was performed using MEGAN (“blastx” analysis) (gray = total reads; orange = domain Bacteria; light-pink = domain Archaea; dark-green = Aquificales (primarily Sulfurihydrogenibium-like); light-green/yellow = class Thermodesulfobacteria; bronze = phylum Korarchaeota; white = class Thermoprotei; dark-blue = Pyrobaculum-like; light-blue = Thermofilum-like; other minor assignments with <100 total reads are not shown).