Figure 2.

Effects of curcumin on Rh123 retention (a) and accumulation (b) in HCT-8/VCR cells. a. The cells were incubated with Rh123 for 60 min, washed, and resuspended in medium with 10 μM curcumin or the vehicle control (0.1% DMSO) for 60 min. Rh123 fluorescence was measured using FACScan. b. The cells were treated with 25 μM curcumin or the vehicle control (0.1% DMSO). Rh123 (1 μg/ml) was added, and the cells were incubated for 120 min. Rh123 fluorescence was measured using flow cytometry. Data are presented as the mean ± SD (n = 3). A: Blank control group; B: Negative control group; C: Curcumin group. The mean fluorescence intensity of the curcumin group was significantly higher than that of the negative control group.** p<0.01 vs. negative control group.