Figure 3.

Inhibition of the expression of MDR1 mRNA and P-gp and survivin mRNA and protein in HCT-8/VCR cells and xenograft tumors. A and B, MDR1 mRNA and P-gp protein expression in HCT-8/VCR cells. 1: control group, 2: curcumin group. RT-PCR showed significant reduction of MDR1 mRNA in curcumin-treated cells. P-gp protein levels detected by western blotting in the above-mentioned groups were consistent with the mRNA expression levels. C, D and E, MDR1 mRNA and P-gp and survivin mRNA and protein expression in xenograft tumors. 1: control group; 2: VCR group; 3: curcumin group; 4: curcumin+VCR group. (C) and (D): RT-PCR showed significant reduction of survivin mRNA and MDR1 mRNA in tumors treated with curcumin or curcumin+VCR. (E) Survivin and P-gp protein levels determined by western blotting in the above-mentioned groups were consistent with the mRNA expression levels. *: vs. control group, p<0.05, #: vs. curcumin group, p<0.05.