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Cloning of Pgd3. (A) Schematic of the Pgd3 locus; two Mu insertions disrupt the predicted ORF of 6PGDH. (B) Complementation test of pgd3-umu1/+ with pgd3-umu2/+; self-pollinated ears and reciprocal crosses segregate for the pgd3 mutation (arrows). (C) 6PGDH native PAGE activity assays for 16-DAP kernels mutated for 6PGDH isoforms.
