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. 2013 Mar 25;64(8):2231–2242. doi: 10.1093/jxb/ert082

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© The Author(2) [2013].

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2. — 6PGDH native PAGE enzyme activity assays. (A) Wild-type plant tissues from the inbred W22; seed tissues were extracted at 16 DAP and 40 µg total protein was loaded per lane. (B) PGD3 activity is reduced in photosynthetic cells; an emerging leaf was harvested and divided in 3cm increments from the shoot apex (0cm) to the photosynthetic tip (24cm). (C, D) Seed developmental time course from W22 (C) and pgd1;pgd2 double mutant (D) kernels; the W22 lane was extracted at 20 DAP. In B–D, 30 µg total protein was loaded per lane (this figure is available in colour at JXB online).