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. 2013 Apr 1;64(8):2243–2253. doi: 10.1093/jxb/ert084

Table 1.

Primers used in (Q-)RT–PCR.

Gene name Accession no. MBS position and sequence Sequence of gene-specific primer Species
TiMYB2R-1 JX683795 F: 5’-ACGGACAACGAGGTCAAGAAC-3’ Thinopyrum intermedium
R: 5’-GAAATGGGCTCCGTACG-3’
PR1a TC384382 –43 to –39, GGATA F: 5’-CGTGGGTGTCGGAGAAGC-3’ Triticum aestivum
R: 5’-AAGTTGCCTGGCGGGTTG-3’
PR17c TA65181 –58 to –54 GGATA F: 5’-ACGACATCACGGCGAGGT-3’ Triticum aestivum
R: 5’-CACGGGGAAAGAGAGGATGA-3’
nsLTP1 TC411506 –86 to –81(–), CTGTTA F: 5’-ATGCGGGTTGGCGTGAAG-3’ Triticum aestivum
R: 5’-TGTTGCGGTGGTAGGTTGTTG-3’
GST22 TC372250 –211 to –206(–), CCGTTG F: 5’-GGGATTGGGGCAGGAG-3’ Triticum aestivum
R: 5’-TCGGGAGGGAGGAAGC-3’
Chit2 TC426538 No promoter sequence F: 5’-TTCTGGATGACGGCACAAG-3’ Triticum aestivum
R: 5’-CCTTAGTGTGACCAGTCGTTTT-3’
Chit3 TC386649 No promoter sequence F: 5’-TAAGATGAGCCCCTACCTGTTC-3’ Triticum aestivum
R: 5’-GTACACGGCATTTATTTAGTCCC-3’
Actin BE425627 F: 5’-CACTGGAATGGTCAAGGCTG-3’ Triticum aestivum
R: 5’-CTCCATGTCATCCCAGTTG-3’
18SrRNA AY049040 F: 5’-GTGACGGGTGACGGAGAATT-3’ Triticum aestivum
R: 5’-GACACTAATGCGCCCGGTAT-3’
Ggt 18SrRNA FJ771002 F: 5’-CGAACTCGGTCGTTTAGAGG-3’ Gaeumannomyces graminis var. tritici
R: 5’-GGTATGTTCACAGGGGTTGG-3’