Fig. 4.

DCL1 and known miRNAs are expressed during axillary bud outgrowth. (A) Detection of SsDCL1 transcripts through qRT-PCR in 0, 2, and 5 DAP developing axillary buds of sugarcane hybrid SP80-3280. (B) Inferred phylogenetic relationships among SsDCL1 orthologues in rice (Os) and Arabidopsis (At). The p-distances were calculated from amino acid alignments of conserved blocks, and tree topology was inferred with the neighbour-joining method. Only bootstrap values higher than 50% are shown for 1000 replicates. SsDCL1 is underlined. (C) Accumulation of selected mature miRNAs was quantified through stem–loop pulsed qRT-PCR in 0, 2, and 5 DAP axillary buds of sugarcane hybrid SP80-3280. All qRT-PCR experiments used inactive bud (0 DAP) as the reference sample (set to 1.0). Error bars indicate standard deviation of three biological replicates. Distinct letters indicate significant differences at P <0.05 (Student’s t-test). R.E., relative expression.