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. 2013 Apr 5;64(8):2307–2320. doi: 10.1093/jxb/ert089

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© The Author(2) [2013].

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 6. — Spatiotemporal expression patterns of miRNAs in axillary buds of sugarcane. (A, B) A probe of a 3’-labelled LNA-modified oligonucleotide detecting miR159 was hybridized with longitudinal sections of 0 DAP (A) and 5 DAP (B) vegetative buds. (C) A similar probe detecting miR156 was hybridized with longitudinal sections of 0 DAP buds. (D) A scramble-miRNA 3’-labelled LNA probe was used as a negative control. Purple and brown staining show probe localization. Arrows represents meristem positions and arrowheads indicates young leaf primordia. Bars, 10 μm.