Fig. 1.

Maximum parsimony tree showing the relationship of the Pyrinae SFBB genes. Md, Malus domestica; Pp, Pyrus pyrifolia; Pb, Pyrus bretschneideri; Pu, Pyrus ussuriensis; Ps, Pyrus sinkiangensis; Pc, Pyrus communis; Sa, Sorbus aucuparia (sequences are indicated by a filled circle). Bx-cx represents a Bragança S. aucuparia population (B) and the individual name (x), with cx representing the colony type. Numbers below the branches represent bootstrap values above 60. Genes of known location in Malus are: Md-X1, Md-X2, Md-X3, Md-X5, Md-X7, Md-X8, Md-X9, Md-X14, Md-X18, Md-X20, MdSFBB3α, and MdSFBB3β for the haplotype S3; and Md-X6, Md-X10, Md-X12, Md-X13, Md-X15, Md-X16, Md-X17, Md-X19, MdSFBB9α, and MdSFBB9β for the haplotype S9 (Sassa et al., 2007; Minamikawa et al., 2010). Genes of known location in Pyrus are: PpSFBB4-d1, PpSFBB4-d2, and PpSFBB4-u1–PpSFBB4-u4 for haplotype S4; and PpSFBB2-d1–PpSFBB2-d5, and PpSFBB2-u1–PpSFBB2-u5 for haplotype S5 (Okada et al., 2011). GenBank numbers are: Pyrus 1 sequences: AB621615, 293337907, EU979310, EU979317, AB621617, EU979315, EU979311, EU979313, 293337889, 293337854, AB270800, AB621616, 293337905, 293337893, 293337887, EU979316, 293337891, AB270797, 293337911, 293337895, EU979309, 293337909; Pyrus 2 sequences: 293337814, 293337810, AB545981, 293337808, 293337806, 293337804; Pyrus 3 sequences: 293337784, 293337782, 293337780, 293337786, 293337778, EU422961, EU422960, 293337788, 293337776, EU081892, EU422958, AB270799, 293337802, EU081890, AB297937, EU422956, AB297935, 293337798, EU418249, EU979327, AB297939, AB297934, AB297938, EU081887, AB297933, 293337796, EU418248, AB297940, AB270802, EU081894, 293337800, EU081891, AB297936, EU081893, EU422959, EU422962; Pyrus 4 sequences: EU979319, EU979320, EU979323, 293337878, 293337844, EU979324. Pyrus 5 sequences: AB621602, 293337876, 293337858, 293337846, 293337842; Malus 1 sequences: 293337901, 293337897, AB539848, 293337903, 293337899, AB539858, FJ008668.