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. 2013 Apr 18;64(8):2499–2510. doi: 10.1093/jxb/ert108

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© The Author(2) [2013].

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 1. — Subcellular localization of MaERFs in tobacco BY-2 protoplasts. Protoplasts were transiently transformed with MaERF–GFP constructs or GFP vector using a modified PEG method. GFP fluorescence was observed with a fluorescence microscope. VirD2NLS-mCherry was included in each transfection to serve as a control for successful transfection, as well as for nuclear localization. Images were taken in a dark field for green fluorescence, while the outline of the cell and the merged were photographed in a bright field. Bars, 25 μm (this figure is available in colour at JXB online).