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. 2013 Apr 18;64(8):2499–2510. doi: 10.1093/jxb/ert108

Fig. 6.

Fig. 6.

Physical interactions between MaERF9 and -11 and MaACS1 and MaACO1 detected in an Y2H assay. The coding regions of MaERF9 and -11 were cloned into the pGADT7 vector to create the AD-MaERF9 and -11 constructs, while the coding regions of MaACS1 and MaACO1 were cloned into the pGBKT7 vector to create the DBD-MaACS1/MaACO1 constructs. Gold Y2H yeast strains were co-transformed with DBD-MaACS1/MaACO1 and AD-MaERF9 and -11, respectively. The ability of yeast cells to grow on synthetic medium lacking tryptophan, leucine, histidine, and adenine but containing 125 μM aureobasidin A and to turn blue in the presence of the chromagenic substrate X-α-Gal was scored as a positive interaction. Yeast cells transformed with pGBKT7-53 + pGADT7-T, DBD–MaACS1 + pGADT7-T, DBD–MaACO1 + pGADT7-T, or pGBKT7-Lam + pGADT7-T were included as positive or negative controls, respectively (this figure is available in colour at JXB online).