Table 2.
Mean values and statistical significance of recordings with blunted voltage-operated Ca2+ channels (VOCC) and voltage-gated Na+ channels (VGNC)
| 8 nM HlgC/HlgB | Mean [Ca2+] peak ± SEM | Latency s ± SEM | Pairwise comparison of mean latencies (Holm-Sidak method) |
|---|---|---|---|
| a: control (65 cells) | 270 ± 15 nM | 210 ± 20 s | a versus b: P < 0.001; a versus c: P < 0.001; a versus d: P < 0.001; a versus e: P < 0.001 |
| b: blockade of L-, N- and P/Q-Type VOCC by 5 μM nifedipine + 0.1 μM ω-GVI-A + 0.1 μM ω-Aga-TK (88 cells) | 265 ± 12 nM | 116 ± 4 s | b versus c: P < 0.001; b versus d: P < 0.001; b versus e: P = 0.839 |
| c: blockade of VGNC by 0.3 μM TTX (24 cells) | 263 ± 22 nM | 436 ± 8 s | c versus d: P < 0.001; c versus e: P < 0.001 |
| d: blockade of L- and N-Type VOCC, VGNC and Glu-R by 5 μM nifedipine + 0.1 μM ω-GVI-A + 0.3 μM TTX + 10 μM CNQX + 10 μM D-AP5 (26 cells) | 196 ± 10 nM | 853 ± 14 s | d versus e: P < 0.001 |
| e: blockade of L-, N- and P/Q-Type, VGNC and Glu-R: 5 μM nifedipine + 0.1 μM ω-GVI-A + 0.1 μM ω-Aga-TK + 0.3 μM TTX + 10 μM CNQX + 10 μM D-AP5; 21 cells | 232 ± 14 nM | 111 ± 6 s |
Mean values and statistical significance of peak amplitudes and latencies of the experiment shown in Fig. 4. The pairwise comparison between the means of the peak amplitude did not reveal significant statistical differences, whereas mean latencies were significantly different, but for experiments b and e only.