Figure 1. The administration of chNKG2D-expressing T cells increases antigen-specific CD4⁺ T cells in the peritoneum and tumor-draining lymph nodes. (A and B) Mice bearing ID8-GFP-tOVA- (A) or ID8-GFP-derived (B) tumors were injected with chNKG2D-expressing, wtNKG2D-expressing T cells or PBS i.p. One hour before local T-cell injection, CD45.2⁺ OT-II cells were injected i.v. Three days after T-cell transfer, the absolute number of CD45.2⁺ cells in the peritoneum and mediastinal lymph nodes was determined by flow cytometry. The average of each group and SD (n = 8) is shown (**p < 0.01; ***p < 0.001 as compared with animals receiving wtNKG2D-expressing cells).