Figure 7. Host CD8⁺ T cells are necessary for complete tumor elimination mediated by chNKG2D-expressing T cells. (A and B) C57BL/6 and Cd8^−/− mice bearing 5-week ID8 tumors were treated with wtNKG2D-expressing or chNKG2D-expressing T cells. (A) Eight weeks after tumor-cell inoculation, the number of solid tumors on the peritoneal wall and number of tumor cells in the peritoneal wash was assessed. (B) Spleen and peritoneal cells were isolated from mice 8 weeks after tumor cell injection and cultured for 24 h. Cell-free supernatants were then assessed for interferon γ (IFNγ) production. The average of each group and SD (n = 8) are shown (***p < 0.001 as compared with C57BL/6 mice receiving wtNKG2D-expressing cells; ^†p < 0.05 as compared with C57BL/6 mice receiving chNKG2D-expressing cells).