Figure 2. A CD14⁺BDCA3⁺ cell population exhibiting macrophage-like morphology and phenotype arises during the maturation of monocyte-derived dendritic cells. (A) Expression of CD1a, CD14, CD83 and BDCA3 on immature monocyte-derived dendritic cells (MoDCs), MoDC matured for 2 d by a mixture of prostaglandin E₂ (PGE₂), tumor necrosis factor α (TNFα), interleukin (IL)-1β and IL-6 (mDCs), and DCs matured in the presence of IL-10 (IL-10-mDCs). (B) Light microscopic images of mDCs and FACS-sorted CD14⁺ and CD14⁻ IL-10-mDCs (400× magnification). (C) Percentages of CD14⁺ DCs matured in the presence (IL-10-mDCs) or absence (mDCs) of IL-10. **p < 0.01 in a Wilcoxon Signed Rank paired analysis. (D) Expression of CD83, CD40, CD80, CD86, B7-H1, HLA-DR, CD16 and DC-SIGN on the indicated mDC subsets. Data are representative of at least eight independent experiments.