Table 3.
Combinations of markers successfully amplified with our one-step multiplex PCR protocol on a single cell, after the update of the method.
| β-Globin | HL A | ||||||
|---|---|---|---|---|---|---|---|
| 5′Region | 3′Region | Upstream HLAA | HLAA-HLAB | HLAB-HLADRA | HLADRA-HLADQB1 | Downstream DQB1 | |
| 1 | D11S1338 | D11S2351 | D6S1624 | HLAC-CA | MIB | DQCAR | RING3-CA |
| 2 | D11S1338 | D11S2351 | MOG-CA | D6S265 | D6S273 | DRA-CA | TAP1A-CA |
| 3 | D11S4891 | D11S4181 | D6S1624 | HLAC-CA | MIB | DRA-CA | TAP1A-CA |
| 4 | D11S1760 | D11S4181 | MOG-CA | D6S265 | D6S273 | DQCAR | RING3-CA |
| 5 | D11S4891 | D11S1871 | D6S1571 | D6S265 | MIB | D6S2443 | D6S1583 |
| 6 | D11S1760 | D11S1871 | MOG-TAAA | HLAC-CA | D6S273 | D6S2443 | D6S1610 |