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. 2013 Apr 21;2013:759057. doi: 10.1155/2013/759057

Figure 2.

Figure 2

Human hepatocellular carcinoma cell line characterization. (a) Assessment of EGFR expression in HCC cell lines, including Hep3B, PLC/PRF/5, and HepG2 cells. Protein ladders are indicated in kDa. Bands for EGFR and β-actin are indicated by arrowheads. (b) EGFR expression in HCC cell lines at mRNA level was assessed by quantitative real-time PCR. Relative quantification of EGFR RNA expression was validated by 18S RNA. (c) Immunofluorescence staining of Hep3B, PLC/PRF/5, and HepG2 cells using FITC labeled anti-EGFR antibody. Positive staining of EGFR was shown in red. Nuclei staining using DAPI is shown in blue. (d) Immunofluorescence staining of Hep3B, PLC/PRF/5, and HepG2 cells using Alexa680-ZEGFR:1907. Staining using unlabeled Ac-Cys-ZEGFR:1907 as a blocking reagent was also shown. Positive staining of EGFR was shown in red. Nuclei staining using DAPI is shown in blue.