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. 2013 Apr 21;2013:759057. doi: 10.1155/2013/759057

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Copyright © 2013 Ping Zhao et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Immunofluorescence and immunohistochemistry staining on subcutaneous HCC xenografts. (a) Immunofluorescence staining of Hep3B, PLC/PRF/5, and HepG2 xenografts using Alex680-Z_EGFR:1907. (b) Immunohistochemistry (IHC) staining on paraffin-embedded subcutaneous tumor tissues, including Hep3B, PLC/PRF/5, and HepG2 tumors. Anti-EGFR antibody was used in 1 : 200 dilution. Nuclei staining was performed using Dako Cytomation Mayer's hematoxylin histological staining reagent. (c) Representative photograph of PLC/PRF/5, Hep3B, and HepG2 subcutaneous tumors.