FIGURE 1.

Example of embryos oriented in agarose. (A) Using standard molds and LMP agarose, numerous embryos can be positioned in the same block. Orientation with respect to the bottom of the mold typically yields the best results, because the surface is flat and can easily be attached to sectioning plates. (B) Once blocks are cooled, excess agarose can be cut away, leaving less material to section and decreasing the time spent sectioning.