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. Author manuscript; available in PMC: 2013 May 15.
Published in final edited form as: Cold Spring Harb Protoc. 2010 Dec 1;2010(12):pdb.prot5532. doi: 10.1101/pdb.prot5532

FIGURE 3.

FIGURE 3

Detection of mRNA and protein in the same section. (A) Embryos that underwent whole-mount in situ hybridization with an antisense probe to the melanocyte marker Trp-2 were sectioned using the method described. Blue arrows indicate signal revealing the position of melanocytes. (B) The sections were processed for immunohistochemistry with an antibody to the phosphorylated form of Histone 3B (a mitosis marker) and an Alexa-555-conjugated secondary antibody (red signal on section). (C) The two images can be easily overlaid, allowing simultaneous detection of both signals, in this case revealing what percentage of mitotic cells is Trp-2-positive. (For color figure, see doi: 10.1101/pdb.prot5532 online at www.cshprotocols.org.)