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. Author manuscript; available in PMC: 2013 May 15.
Published in final edited form as: Cold Spring Harb Protoc. 2010 Dec 1;2010(12):pdb.prot5532. doi: 10.1101/pdb.prot5532

Table 2.

Sample secondary antibodies that can be used in Xenopus

Detection Source information Dilution
Fluorescence Invitrogen Alexa Fluor 546 or Alexa Fluor 555, anti-rabbit or anti-mouse 1:500
Alkaline phosphatase Jackson ImmunoResearch 1:1000–1:2500
HRP (horseradish peroxidase) Jackson ImmunoResearch 1:750 using TrueBlue peroxidase substrate (KPL)