Table 2.
Detection | Source information | Dilution |
---|---|---|
Fluorescence | Invitrogen Alexa Fluor 546 or Alexa Fluor 555, anti-rabbit or anti-mouse | 1:500 |
Alkaline phosphatase | Jackson ImmunoResearch | 1:1000–1:2500 |
HRP (horseradish peroxidase) | Jackson ImmunoResearch | 1:750 using TrueBlue peroxidase substrate (KPL) |