| Immunoprecipitate (“IP” fraction) | 1.25 μl |
| Taq PCR buffer | 5 μl |
| dNTP’s (2.5 mM stock) | 1.6 μl |
| Forward primer (10 mM) | 1 μl |
| Reverse primer (10 mM) | 1 μl |
| Taq polymerase | 0.2 μl |
| Water | 15 μl |
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