Figure 6.

Genes differentially regulated ins-l flies are modulated by sleep loss in humans. A, Filamin-A (Cheerio) and Malic enzyme (Men) are both upregulated in ins-l^w flies compared with ns^w flies. QPCR data from 20 fly heads collected at ZT0. Levels are expressed as percentage of ns^w expression. B, Filamin-A and Malic enzyme expressions are elevated after 28 h of waking in humans (n = 8; Wilcoxon signed rank test, p = 0.02 and p = 0.059, respectively). QPCR data obtained from saliva mRNA extracts. Each saliva sample collected after sleep deprivation was compared with a circadian matched baseline sample from the same subject. Levels are expressed as percentage of baseline expression. Error bars represent SEM.