BDNF expression at 30 minutes and 24 hours after an acute administration of Yueju. (a) BDNF and BDNF precursor (pro-BDNF) protein expression levels in mouse hippocampus were determined by Western blot at 30 minutes after treatment with Yueju (YJ) or ketamine (KET). For BDNF, one-way ANOVA, F
(2,15) = 9.687; P = 0.002. For pro-BDNF, one-way ANOVA, F
(2,15) = 9.302; P = 0.002. (b) BDNF and pro-BDNF protein expression levels at 24 hours after drug treatment. For BDNF, one-way ANOVA, F
(2,15) = 18.81; P = 0.003. For pro-BDNF, one-way ANOVA, F
(2,15) = 9.416; P = 0.014. (c) BDNF mRNA expression was examined by quantitative RT-PCR in mouse hippocampus at 30 min after drug treatment. One-way ANOVA, F
(2,15) = 0.3193; P = 0.736. *P < 0.05; **P < 0.01, compared with the control group (CTL). Data represent mean ± SEM. n = 6.