Figure 2. The j-factors of short DNA fragments. The dashed and dotted lines correspond to the calculated j-factors for arbitrary angles between the duplex ends and capture radius, rc, of 3 nm (dotted line) and 5 nm (dashed line). The points correspond to the experimental data shown in Figure 3E of V&H. The thin black line corresponds to the j-factor that accounts for axial and torsional orientations of the fragment ends. All calculated curves correspond to DNA persistence length of 48 nm. The calculations were made as described in ref. 12.