Figure 4.

Quantitative expression of genetic markers for adherens junction and intracellular scaffolding proteins. RT-PCR of human genes for E-cadherin (CDH1), β-catenin (CTNNB1), and β-actin (ACTB) in untreated and CdtABC-treated HGX (eight individuals) and primary HGEC (six individuals). Untreated, white bars; CdtABC-treated (10 μg/mL), black and patterned bars. Note that the data are plotted with difference scales on the Y-axes. Mean values ± SD are shown (n = 3 in each group). Asterisks mark statistically significant differences (two-sample unequal variance test) between the relative expression levels of mRNA from untreated and CdtABC-treated HGX or HGEC (*p ≤ .05, **p ≤ .1). Experiments were performed a minimum of 3 times.