Figure 1. Metagene analyses. The relative distribution of the indicated proteins around the average transcription start site (TSS) for a custom set of genes in which genes with TSSs within 1000 bp of another were excluded (20,799 mouse genes or 20,286 human genes). The profiles were generated for regions -10 kb to + 10 kb, backgrounds were subtracted, and the curves were normalized as described earlier.12 Relative amplitudes within a given curve or between curves can be compared but absolute amplitudes between curves should not be compared. Cell types: human cervical cancer cells (HeLa), human fibroblasts (IMR90), mouse embryonic stem cells (MES), mouse embryonic fibroblasts (MEF). Data sets analyzed: human (GSE35886),12,13 MEF (GSE24113, GSE22562),19,20 MES (GSE20530, GSE22562, GSE22303).8