Figure 1. (A) Organization of centromere DNA in different eukaryotes. (B) Effects of CBF1, STE12 or DIG1 deletion and overexpression from the MET25 promoter on centromere transcription. High or low levels of centromere transcription, such as MET25 promoter and cbf1∆ or ste12∆ cells, impair centromere function. The proper, intermediate, level of transcription is indicated by the green bar. (C) Schematic representation of the organization of putative PREs in the pericentromeric region of CEN3. Numbers indicate the spacing in nucleotides between adjacent PREs.