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. Author manuscript; available in PMC: 2014 May 28.
Published in final edited form as: Org Biomol Chem. 2013 May 28;11(20):3341–3348. doi: 10.1039/c3ob40395e

Figure 6.

Figure 6

Irreversibility of mitochondrial fragmentation by GA and CLV (a-f) Cells were assessed by IF for their ability to regain mitochondrial integrity after 90 min of recovery in fresh medium following treatment with GA for 0 min (a), 15 min (b) and 30 min (c) or CLV for 0 min (d), 15 min (e) and 30 min (f). Recovery after 30 min of treatment for both GA (c) and CLV (f) could not restore mitochondrial integrity. Mitochondria are shown in red; nuclei are in blue. (g-h) Western Blot analysis was used to measure the degree of irreversibility by caspase-3 (C3) activation. As shown in (g) (GA) and (h) (CLV), while incubation times shorter than 90 min did not induce C3 activation (lanes 1-4 and lanes 10–12), after recovery in the absence of the drugs, C3 was shown to be active once the compounds had been present on the cells for more than 30 min (lanes 7–9 and 15–17). Tubulin (bottom line) was used as loading control.