Table 1.
MS conditions for the CYP metabolites and internal standards
| Enzyme | Metabolites | Precursor ion mass (m/z) | Product ion mass (m/z) | Ionisation Mode | Cone voltage (V) | Collision energy (eV) | Retention time (min.) |
|---|---|---|---|---|---|---|---|
| CYP1A2 | Acetaminophen | 152.03 | 110.03 | ESI + | 25 | 15 | 4.26 |
| CYP2C9 | 4′-Hydroxy diclofenac | 311.82 | 229.97 | ESI + | 25 | 32 | 5.24 |
| CYP2C19 | 4-Hydroxy mephenytoin | 234.91 | 132.77 | ESI + | 30 | 20 | 4.64 |
| CYP2D6 | Dextrorphan | 257.98 | 156.96 | ESI + | 30 | 30 | 4.29 |
| CYP3A4 | 1′-Hydroxy midazolam | 341.91 | 323.85 | ESI + | 20 | 25 | 5.09 |
| Enzyme | Internal Standards | Precursor ion mass (m/z) | Product ion mass (m/z) | Ionisation mode | Cone voltage (V) | Collision energy (eV) | Retention time (min.) |
|---|---|---|---|---|---|---|---|
| CYP1A2 | Acetaminophen-D4 | 155.71 | 113.86 | ESI + | 25 | 15 | 4.26 |
| CYP2C9 | 4′-Hydroxy Diclofenac-D4 | 315.82 | 234.00 | ESI + | 25 | 32 | 5.24 |
| CYP2C19 | Mephenytoin-d3 | 237.88 | 150.03 | ESI + | 30 | 20 | 4.64 |
| CYP2D6 | Dextrorphan-d3 | 261.00 | 244.00 | ESI + | 30 | 30 | 4.29 |
| CYP3A4 | Midazolam-d5 | 330.76 | 295.77 | ESI + | 20 | 25 | 4.79 |