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. Author manuscript; available in PMC: 2013 Sep 25.
Published in final edited form as: ACS Nano. 2012 Jul 27;6(9):8209–8215. doi: 10.1021/nn302896c

Figure 1.

Figure 1

Assembling integrated DNA origami structures from both strands of a dsDNA scaffold. A. The assembly process: A double stranded DNA template is isolated by gel electrophoresis and denatured by heating at 90°C for 15 minutes. The scaffold strands (blue and red) are subsequently folded by a collection of short oligonucleotides staples present in large excess with rapid cooling and re-annealing from a moderate temperature (See SI for details). The arrows indicate the temperature changes. B. Top row: Schematic diagrams of a square, triangle, and 24-helix bundle structure, assembled using λ5 or λ6 (2322 bps and 2027 bps, respectively) dsDNA as the scaffold. B. Bottom row: AFM images of the assembled structures. Scale bars are 100 nm. The Inset in each image panel shows a zoom in image.