TABLE I.
Cytokine concentration (ng/ml) | MDA-231W-GFP Pre | MDA-231W-GFP Post | MDA-231PY-GFP Pre | MDA-231PY-GFP Post | MDA-231BO-GFP Pre | MDA-231BO-GFP Post | MDA-231BRMS-GFP Pre | MDA-231BRMS-GFP Post |
---|---|---|---|---|---|---|---|---|
Human metastatic breast cancer cell variant type | ||||||||
IL-6 | 1.5 (1.5, 1.5) | 0.7 (0.7, 0.7) | 0.2 (0.2, 0.2) | 0.09 (0.03, 0.3) | 0.4 (0.4, 0.4) | 0.09 (0.05, 0.2) | 0.9 (0.9, 0.9) | Did not grow |
GRO-a | 3.5 (3.5, 3.5) | 0.5 (0.5, 0.5) | 0.3 (0.3, 0.3) | 0.4 (0.4, 0.4) | 1.7 (1.7, 1.7) | 0.4 (0.3, 0.6) | 2.4 (2.4, 2.4) | Did not grow |
MCP-1 | 0.04 (0.04, 0.04) | 0.02 (0.02, 0.02) | 0.06 (0.06, 0.06) | 0.04 (0, 0.09) | 0.05 (0.05, 0.05) | 0.03 (0.03, 0.03) | 0.02 (0.02, 0.02) | Did not grow |
IL-8 | 2.3 (2.3, 2.3) | 0.6 (0.6, 0.6) | 0.5 (0.5, 0.5) | 0.5 (0.3, 0.8) | 2.6 (2.6, 2.6) | 0.8 (0.4, 1.9) | 2.3 (2.3, 2.3) | Did not grow |
VEGF | 13.0 (13.0, 13.0) | 7.9 (7.9, 7.9) | 13.0 (13.0, 13.0) | 9.9 (5.9, 16.0) | 27.0 (27.0, 27.0) | 9.0 (8.3, 9.8) | 9.8 (9.8, 9.8) | Did not grow |
The cytokine concentration of pre-inoculated and retrieved human metastatic breast cancer cell variants was determined using a Bio-Rad Bio-Plex™ human cytokine array. Geometric mean (geometric mean −1 SD, geometric mean +1 SD), values were rounded to the nearest integer. Compilation of cells from at least two mice per region. No significant differences were found (P > 0.05)