Table 3.
Functional enrichment of differentially expressed transcripts among three samples
| Sub-ontology | GO terms | Reference | 0 vs 2 | pval | p.adj |
|---|---|---|---|---|---|
| Biology process |
primary metabolic process |
11,575 |
445 |
0.00011 |
0.00399 |
| |
interspecies interaction between organisms |
77 |
10 |
0.00022 |
0.00784 |
| cellular component |
intracellular part |
12,026 |
524 |
5.66E-05 |
0.00125 |
| |
membrane-bounded organelle |
9,551 |
432 |
0.00017 |
0.00363 |
| |
intracellular organelle |
10,868 |
473 |
0.00051 |
0.01122 |
| molecular function |
transferase activity |
5,774 |
298 |
1.91E-09 |
2.87E-08 |
| |
|
|
|
|
|
| Sub-ontology |
GO terms |
Reference |
0 vs 24 |
pval |
p.adj |
| Biology process |
primary metabolic process |
11,575 |
994 |
1.71E-09 |
6.15E-08 |
| |
cellular metabolic process |
8,913 |
753 |
0.00046 |
0.01653 |
| cellular component |
membrane-bounded organelle |
9,551 |
883 |
8.48E-07 |
1.87E-05 |
| |
intracellular part |
12,026 |
1,037 |
3.97E-05 |
0.00087 |
| |
intracellular organelle |
10,868 |
947 |
0.00016 |
0.00360 |
| |
vesicle |
1,648 |
181 |
0.00171 |
0.03755 |
| molecular function |
transferase activity |
5,774 |
579 |
1.19E-12 |
1.79E-11 |
| |
hydrolase activity |
5,415 |
490 |
0.00018 |
0.0027 |
| |
nucleotide binding |
5,540 |
490 |
0.00092 |
0.01375 |
| |
|
|
|
|
|
| Sub-ontology |
GO terms |
Reference |
2 vs 24 |
pval |
p.adj |
| Biology process |
primary metabolic process |
11,575 |
866 |
4.61E-09 |
1.66E-07 |
| |
cellular metabolic process |
8,913 |
649 |
0.00136 |
0.04901 |
| cellular component |
intracellular part |
12,026 |
889 |
7.02E-05 |
0.00154 |
| |
intracellular organelle |
10,868 |
805 |
0.00068 |
0.01499 |
| molecular function |
transferase activity |
5,774 |
506 |
6.51E-12 |
9.76E-11 |
| hydrolase activity | 5,415 | 458 | 6.68E-08 | 1.00E-06 |
GO annotation information of differentially expressed transcripts was clustered into three sub-ontologies, including molecular function, biological process, and cellular component [59]. Then hypergeometric test were performed with threshold value of padj ≤0.05 in each sub-ontology to indentify significant enriched GO terms. Reference means the transcripts annotated to the sub-ontology in the whole transcriptome.