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Phenotype of the constructed S. cerevisiae strains. Cultures of S. cerevisiae strains, carrying the genes shown, were grown in A) SC medium with 300 μM CuSO4 and without uracil, histidine, tryptophan and leucine. Cells were grown for 4 days at 30°C, 160 rpm shaking, and collected by centrifugation. B) SC agar plates with 500 μM CuSO4 and without uracil, histidine, tryptophan and leucine. Grown for 4 days at 30°C. The strains accumulated the indicated rubrofusarin pathway products.
