Figure 4.

Detection of intermediates inside and outside of the cells. Detection of the rubrofusarin pathway intermediates in the constructed S. cerevisiae strains, shown by mean areas of the integrated peaks from extracted ion chromatograms for A) YWA1, B) Nor-rubrofusarin, and shown by mean titer (mg/L) calculated for C) Rubrofusarin. Measurements performed for the analyzed cell pellet (C) and growth medium (GM) of the constructed S. cerevisiae strains (see Table 1). Error bars indicate the std. deviation of each integrated peak (n = 3). Note that the mean areas are not comparable between different compounds.