Table 1.
S. cerevisiae strains used in the study
| Strain | Plasmids carried | Relevant genotype |
|---|---|---|
|
S. cerevisiae control |
pRS416 (URA3) |
- |
| pRS413 (HIS3) | ||
| pRS414 (TRP1) | ||
| pRS415 (LEU2) | ||
|
S. cerevisiae PKS12/npgA |
pRS416::pCUP1-PKS12-tADH1, |
PKS12, npgA |
| pRS413::pPYK1-npgA-tTEF1 | ||
| pRS414 | ||
| pRS415 | ||
|
S. cerevisiae PKS12/npgA/aurZ |
pRS416::pCUP1-PKS12-tADH1, |
PKS12, npgA, aurZ |
| pRS413::pPYK1-npgA-tTEF1 | ||
| pRS414::pTEF1-aurZ-tENO2 | ||
| pRS415 | ||
|
S. cerevisiae PKS12/npgA/aurJ |
pRS416::pCUP1-PKS12-tADH1, |
PKS12, npgA, aurJ |
| pRS413::pPYK1-npgA-tTEF1 | ||
| pRS415::pGPD1-aurJ-tCYC1 | ||
| pRS414 | ||
| S. cerevisiae PKS12/npgA/aurZ/aurJ | pRS416::pCUP1-PKS12-tADH1, |
PKS12, npgA, aurZ, aurJ |
| pRS413::pPYK1-npgA-tTEF1 | ||
| pRS414::pTEF1-aurZ-tENO2 | ||
| pRS415::pGPD1-aurJ-tCYC1 |
S. cerevisiae strains that were analyzed for production of rubrofusarin and precursors. Final plasmids transformed into S. cerevisiae strains showing the vector backbone in which the constructed promoter (p) – ORF – terminator (t) gene cassettes were inserted. The auxotrophic marker gene is also shown. All plasmids were maintained by the low copy number sequences ARS/CEN.